Journal: PeerJ

Article Title: ULK2 suppresses ovarian cancer cell migration and invasion by elevating IGFBP3.

doi: 10.7717/peerj.17628

Figure Lengend Snippet: Figure 4 The regulation of IGFBP3-mediated insulin pathway by ULK2. (A) Venn diagram summarizing the results of RNA sequencing; (B) Volcano plot displaying the differential gene expression; (C) Heatmap illustrating the significant differential gene expression identified through RNA sequencing; (D) IGFBP3 expression level was detected by Western blot; β-actin was used as the internal control. (E and F) Gene set enrichment analysis (GSEA) enrichment analysis. Full-size

Article Snippet: IGFBP3 antibody was purchased from cell signaling technology (25864, MA, USA). β-actin from Proteintech (66009-1-Ig; Wuhan, Hubei, China) was utilized as the internal control.

Techniques: RNA Sequencing, Gene Expression, Expressing, Western Blot, Control